Application of Gas Chromatography in Biological Analysis

1. Analysis of human anaphylaxis product Fluorine ethylene biological monitoring indicators - determination of sulphodiacetic acid in urine. The use of vinyl chloride monomer as a raw material for the production of PVC was found to be detrimental to health. The biological monitoring of vinyl chloride was studied. From its metabolism in the body, it was found to be in the form of Sulfur Diacetic Acid. In the middle discharge, taking urine samples from workers exposed to vinyl chloride, methyl esterification can be determined by gas chromatography (DMCS). Using a 2m long, 3mm internal diameter glass column filled with 5% PEG20M/ChromoworbW, AW60~80 mesh; column temperature 180°C, vaporization and detection chamber 201°C; high purity nitrogen as carrier gas 50ml/min; flame luminosity Detector, hydrogen 60 ml/min, air 70 ml/min. The urine was acidified and double-esterified to become a dithiol diacetate, concentrated to 1 ml, 2 μl was taken into the chromatographic system, and a peak of the dithiodiacetate was obtained at 5 minutes.

2. Drug analysis For example, barbiturate hypnotics analysis, using gas chromatography to complete the quantitative analysis of a variety of barbiturates hypnotics. Barbiturates were first methylated and chromatographed. The diazomethane method is a relatively simple method for the methylation of barbiturate hypnotics, and more than 95% of them produce N,N dimethyl barbiturates hypnotics. With 2% OV-17, 5% SE-30 and other high-temperature fixing solution can be isolated on the OV-17 column derivative spectra.